Employee profile

PhD Ewa Niedziałkowska

Employee since 2014

Room: 234

Phone: +48 12 6395 158

E-mail: nceniedz(at)cyf-kr.edu.pl

Research topics:

Structure-function relationship of proteins

Methodology:

X-ray crystallography

Isothermal Titration Calorimetry (ITC)

Fluorescence anisotropy

FPLC

Molecular genetics

Achievements:

Publications:

Niedzialkowska, E., Wang, F., Porebski, P. J., Minor, W., Higgins, J. M. & Stukenberg, P. T. (2012). Molecular basis for phosphospecific recognition of histone H3 tails by Survivin paralogues at inner centromeres. Molecular Biology of the Cell 23, 1457-1466.



Szpak, K., Wybieralska, E., Niedzialkowska, E., Rak, M., Bechyne, I., Michalik, M., Madeja, Z. & Czyz, J. (2011).

DU-145 prostate carcinoma cells that selectively transmigrate narrow obstacles express elevated levels of Cx43. Cellular & Molecular Biology Letters 16, 625-637.



Wang, F., Dai, J., Daum, J. R., Niedzialkowska, E., Banerjee, B., Stukenberg, P. T., Gorbsky, G. J. & Higgins, J. M. (2010). Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis. Science 330, 231-235.



Kaczmarek, K., Niedzialkowska, E., Studencka, M., Schulz, Y. & Grzmil, P. (2009). Ccdc33, a predominantly testis-expressed gene, encodes a putative peroxisomal protein. Cytogenetic and Genome Research 126, 243-252



PDB deposits:

3UED, 3UEE, 3UEF,3UEG,3UEH,3UEI, 4IW7, 4OAD, 3V48, 3V4D, 3OT1, 3UEC, 4IQG, 4JXU, 3HHL, 4GQA, 4K2H

2018

  • A. Kluza, E. Niedzialkowska, K. Kurpiewska, Z. Wojdyla, M. Quesne, E. Kot, P.J. Porebski, T. Borowski, "Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway", Journal of Structural Biology, (2018) 229-235

2017

  • A. Rugor, M. Tataruch, J. Staroń, A. Dudzik, E. Niedzialkowska, P. Nowak, A. Hogendorf, A. Michalik-Zym, D. B. Napruszewska, A. Jarzębski, K. Szymańska, W. Białas, M. Szaleniec, "Regioselective hydroxylation of cholecalciferol, cholesterol and other sterol derivatives by steroid C25 dehydrogenase", Applied Microbiology and Biotechnology, 101 (2017) 1163-1174
  • E. Niedzialkowska, B. Mrugala, A. Rugor, P.C. M, A. Skotnicka, J.J. Cotelesage, G.N. George, M. Szaleniec, W. Minor, K. Lewinski,, "Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterization", Protein Expr. Purif., 134 (2017) 47-62
  • A. Rugor, A. Wójcik-Augustyn, E. Niedzialkowska, S. Mordalski, J. Staroń A. Bojarski, M. Szaleniec, "Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase – homology model, reactivity and isoenzymatic diversity", J. Inorg. Biochem., 173 (2017) 28–43

2016

  • E. Niedzialkowska, O. Gasiorowska, K.B. Handing, K.A. Majorek, P.J. Porebski, I.G. Shabalin, E. Zasadzinska, M. Cymborowski, W. Minor, "Protein purification and crystallization artifacts: The tale usually not told.", Protein Sci., 10.1002/pro.2861
  • M. Grabowski, E. Niedzialkowska, M. D. Zimmerman, W. Minor , "The impact of structural genomics: the first quindecennial ", J Struct Funct Genomics. , 10.1007/s10969-016-9201-5