Employee profile

Mgr Ewa Kot

Employee since 10

Room: 223, 224, 225, 226, 234

Phone: +48 12 6395 158, 18

E-mail: ncwierus(at)cyf-kr.edu.pl

Research topics:

The relationship between the structure of the protein and the functions they perform.


Molecular genetics.


Site-directed Mutagenesis.

Methodology:

- protein purification chromatography,


- protein and DNA electrophoresis technique,


- determination of iron and nickel content in proteins,


- genetic engineering methods (gene cloning, bacterial transformation),


- stoichiometry measurements of protein-ligand interactions by microscale thermophoresis (MST),


- differential fluorimetry stability measurements (nanoDSF),


Achievements:

Fellowships:



01.2019 - 04.2019 - research fellowship at the Biotransformation and Biocatalysis Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands

2018

  • A.M. Miłaczewska, E. Kot, J.A. Amaya, T.M. Makris, M. Zając, J. Korecki, A. Chumakov, B. Trzewik, S. Kędracka-Krok, W. Minor, M. Chruszcz, T. Borowski, "On structure and reaction mechanism of human acireductone dioxygenase", CHEM-EUR J, (2018) 5225-5237
  • A. Kluza, E. Niedzialkowska, K. Kurpiewska, Z. Wojdyla, M. Quesne, E. Kot, P.J. Porebski, T. Borowski, "Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway", Journal of Structural Biology, (2018) 229-235
  • E. Kot, K. Kurpiewska, M. Szaleniec, "Czy można ujarzmić ewolucję czyli słów kilka o ukierunkowanej ewolucji", Wszechświat Pismo Przyrodnicze, 119 (10-12) (2018) 221-227