Profil pracownika

Dr Daniel Knack

Pracownik IKiFP PAN od: 2013-2014

Postdoctoral Research Fellow

Pokój:

Tematyka badawcza:

- Investigation of the catalytic properties of novel molybdenum hydroxylases (ethylbenzene dehydrogenase, S25 dehydrogenase)
- Development of an overproduction systems for those enzymes in E. coli

Metodyka:

- Cloning of genes of and construction of bacterial overexpression vectors
- Overexpression of the genes in E. coli
- Purification and characterisation of the recombinant enzymes

Dalsze informacje:

PhD (2013) Philipps University of Marburg, Laboratory for Microbial Biochemistry
M.Sc. (2009) Philipps University of Marburg, Laboratory for Microbial Biochemistry
B.Sc. (2007) Philipps University of Marburg, Laboratory for Cell Biology

Fellowships:

May 2008 - October 2008: BRAIN AG (Zwingenberg/Germany)
Internship in the department of Molecular Cell Biology

2014

  • M. Tataruch, J. Heider, J. Bryjak, P. Nowak, D. Knack, A.Czerniak, J. Liesiene, M. Szaleniec, "Suitability of the hydrocarbon-hydroxylating molybdenum-enzyme ethylbenzene dehydrogenase for industrial chiral alcohol production", J. Biotechnol., 192 (2014) 400-409

2013

  • A. Dudzik, B. Kozik, M. Tataruch, A. Wójcik, D. Knack, T. Borowski, J. Heider, M. Witko, M. Szaleniec, "The reaction mechanism of chiral hydroxylation of p-OH and p-NH2 substituted compounds by ethylbenzene dehydrogenase", Can. J. Chem., 91 (2013) 775-786
  • D. H. Knack, J. L. Marshall, G. P. Harlow, A. Dudzik, M. Szaleniec, S-Y. Liu, J. Heider, "BN/CC isosteric compounds as new class of enzyme inhibitors: N- and B-ethyl-1,2-azaborine inhibit ethylbenzene hydroxylation as non-convertible substrate analogs", Angew. Chem. Int. Ed., 52 (2013) 2599 –2601
  • D. H. Knack, J. L. Marshall, G. P. Harlow, A. Dudzik, M. Szaleniec, S-Y. Liu, J. Heider, "BN/CC-isosterische Verbindungen als Enzyminhibitoren: Hemmung der Hydroxylierung von Ethylbenzol durch N - und B -Ethyl-1,2-aza- borin als nichtkonvertierbare Substratanaloga", Angew. Chem., 125 (2013) 2660 –2662

2012

  • D. Knack, C. Hagel, M. Szaleniec, A. Dudzik, A. Salwinski, J. Heider, "Substrate and inhibitor spectrum of ethylbenzene dehydrogenase: perspectives on application potential and catalytic mechanism", Appl. Environ. Microb., 78 (2012) 6475–6482